Heterologous production of a cyanobacterial bacteriocin with potent antibacterial activity

https://doi.org/10.1016/j.crbiot.2021.10.002Get rights and content
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Highlights

  • Novel peptide antibiotic from a terrestrial cyanobacterium.

  • Successful heterologous production with E. coli.

  • Potent activity against several Gram-positive bacteria.

Abstract

With regard to the emerging health threat by antibiotic resistant bacteria, bacteriocins are considered as a promising way to overcome this urgent problem. In this work, a hitherto unknown bacteriocin from the terrestrial cyanobacterium Chroococcidiopsis cubana was heterologously expressed in Escherichia coli, purified to homogeneity and tested for activity against several bacteria and one yeast species. The compound showed potent bacteriolytic activity against several Gram-positive bacteria and slight activity against one Gram-negative strain. The bacteriocin had no cytotoxic impact on mouse neuroblastoma N2a cells, indicating its potential for treatment against Gram-positive bacterial pathogens in human diseases.

Keywords

Cyanobacteria
Bacteriocins
Chroococcidiopsis cubana

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